Intrinsic motor neuron excitability is increased after resistance training in older adults.

This study investigated the effects of high-intensity resistance training on estimates of the motor neuron persistent inward current (PIC) in older adults. Seventeen participants (68.5 ± 2.8 yr) completed a 2-wk nonexercise control period followed by 6 wk of resistance training. Surface electromyographic signals were collected with two 32-channel electrodes placed over soleus to investigate motor unit discharge rates. Paired motor unit analysis was used to calculate delta frequency (ΔF) as an estimate of PIC amplitudes during 1) triangular-shaped contractions to 20% of maximum torque capacity and 2) trapezoidal- and triangular-shaped contractions to 20% and 40% of maximum torque capacity, respectively, to understand their ability to modulate PICs as contraction intensity increases. Maximal strength and functional capacity tests were also assessed. For the 20% triangular-shaped contractions, ΔF [0.58-0.87 peaks per second (pps); P ≤ 0.015] and peak discharge rates (0.78-0.99 pps; P ≤ 0.005) increased after training, indicating increased PIC amplitude. PIC modulation also improved after training. During the control period, mean ΔF differences between 20% trapezoidal-shaped and 40% triangular-shaped contractions were 0.09-0.18 pps (P = 0.448 and 0.109, respectively), which increased to 0.44 pps (P < 0.001) after training. Also, changes in ΔF showed moderate to very large correlations (r = 0.39-0.82) with changes in peak discharge rates and broad measures of motor function. Our findings indicate that increased motor neuron excitability is a potential mechanism underpinning training-induced improvements in motor neuron discharge rate, strength, and motor function in older adults. This increased excitability is likely mediated by enhanced PIC amplitudes, which are larger at higher contraction intensities.NEW & NOTEWORTHY Resistance training elicited important alterations in soleus intrinsic motor neuronal excitability, likely mediated by enhanced persistent inward current (PIC) amplitude, in older adults. Estimates of PICs increased after the training period, accompanied by an enhanced ability to increase PIC amplitudes at higher contraction intensities. Our data also suggest that changes in PIC contribution to self-sustained discharging may contribute to increases in motor neuron discharge rates, maximal strength, and functional capacity in older adults after resistance training.

Lower motor unit discharge rates in gastrocnemius lateralis, but not in gastrocnemius medialis or soleus, in runners with Achilles tendinopathy: a pilot study.

OBJECTIVES: Deficits in muscle performance could be a consequence of a reduced ability of a motor neuron to increase the rate in which it discharges. This study aimed to investigate motor unit (MU) discharge properties of each triceps surae muscle (TS) and TS torque steadiness during submaximal intensities in runners with Achilles tendinopathy (AT). METHODS: We recruited runners with (n = 12) and without (n = 13) mid-portion AT. MU discharge rate was analysed for each of the TS muscles, using high-density surface electromyography during 10 and 20% isometric plantar flexor contractions. RESULTS: MU mean discharge rate was lower in the gastrocnemius lateralis (GL) in AT compared to controls. In AT, GL MU mean discharge rate did not increase as torque increased from 10% peak torque, 8.24 pps (95% CI 7.08 to 9.41) to 20%, 8.52 pps (7.41 to 9.63, p = 0.540); however, in controls, MU discharge rate increased as torque increased from 10%, 8.39 pps (7.25-9.53) to 20%, 10.07 pps (8.89-11.25, p < 0.001). There were no between-group difference in gastrocnemius medialis (GM) or soleus (SOL) MU discharge rates. We found no between-group differences in coefficient of variation of MU discharge rate in any of the TS muscles nor in TS torque steadiness. CONCLUSION: Our data demonstrate that runners with AT may have a lower neural drive to GL, failing to increase MU discharge rate to adjust for the increase in torque demand. Further research is needed to understand how interventions focussing on increasing neural drive to GL would affect muscle function in runners with AT.

Intrinsic motoneuron excitability is reduced in soleus and tibialis anterior of older adults.

Age-related deterioration within both motoneuron and monoaminergic systems should theoretically reduce neuromodulation by weakening motoneuronal persistent inward current (PIC) amplitude. However, this assumption remains untested. Surface electromyographic signals were collected using two 32-channel electrode matrices placed on soleus and tibialis anterior of 25 older adults (70 ± 4 years) and 17 young adults (29 ± 5 years) to investigate motor unit discharge behaviors. Participants performed triangular-shaped plantar and dorsiflexion contractions to 20% of maximum torque at a rise-decline rate of 2%/s of each participant's maximal torque. Pairwise and composite paired-motor unit analyses were adopted to calculate delta frequency (ΔF), which has been used to differentiate between the effects of synaptic excitation and intrinsic motoneuronal properties and is assumed to be proportional to PIC amplitude. Soleus and tibialis anterior motor units in older adults had lower ΔFs calculated with either the pairwise [-0.99 and -1.46 pps; -35.4 and -33.5%, respectively] or composite (-1.18 and -2.28 pps; -32.1 and -45.2%, respectively) methods. Their motor units also had lower peak discharge rates (-2.14 and -2.03 pps; -19.7 and -13.9%, respectively) and recruitment thresholds (-1.50 and -2.06% of maximum, respectively) than young adults. These results demonstrate reduced intrinsic motoneuron excitability during low-force contractions in older adults, likely mediated by decreases in the amplitude of persistent inward currents. Our findings might be explained by deterioration in the motoneuron or monoaminergic systems and could contribute to the decline in motor function during aging; these assumptions should be explicitly tested in future investigations.

Influence of Trunk Position during Three Lunge Exercises on Muscular Activation in Trained Women.

The present study aimed to compare the activation of the lower lumbar erector spinae, gluteus maximus, biceps femoris, and rectus femoris in two trunk positions (straight, and inclined) during three lunge exercises (static, step-forwarding, and walking) in trained young women in a randomized crossover design. Twelve women (24 ± 3 years) were selected and performed the lunge exercise with an overload of 30% of body weight in six conditions to analyze muscle activation via surface electromyography signals. Higher activation in the erector spinae (%MVIC) were observed (p < 0.05) when trunk position was inclined (straight = 20 ± 15, inclined = 40 ± 29) and during the walking lunge condition (static = 24 ± 16, forward = 26 ± 22, walking = 40 ± 33). Higher activation in the gluteus maximus was observed during step-forward and walking lunges conditions (static = 31 ± 12, forward = 54 ± 20, walking = 58 ± 30). All conditions displayed similar activations in the biceps femoris and rectus femoris (p > 0.05). Results indicate that positioning the trunk in a forward-inclined position induces greater lower lumbar erector spinae activation and dynamic lunge variations elicit greater muscular activation in the gluteus maximus than static lunges. Additionally, it seems that trunk and exercise variations do not influence the activation of tight muscles.

Estimates of persistent inward currents increase with the level of voluntary drive in low-threshold motor units of plantar flexor muscles.

This study tested whether estimates of persistent inward currents (PICs) in the human plantar flexors would increase with the level of voluntary drive. High-density surface electromyograms were collected from soleus and gastrocnemius medialis of 21 participants (29.2 ± 2.6 yr) during ramp-shaped isometric contractions to 10%, 20%, and 30% (torque rise and decline of 2%/s and 30-s duration) of each participant's maximal torque. Motor units identified in all the contraction intensities were included in the paired-motor unit analysis to calculate delta frequency (ΔF) and estimate the PICs. ΔF is the difference in discharge rate of the control unit at the time of recruitment and derecruitment of the test unit. Increases in PICs were observed from 10% to 20% [Δ = 0.6 pulse per second (pps); P < 0.001] and from 20% to 30% (Δ = 0.5 pps; P < 0.001) in soleus and from 10% to 20% (Δ = 1.2 pps; P < 0.001) but not from 20% to 30% (Δ = 0.09 pps; P = 0.724) in gastrocnemius medialis. Maximal discharge rate increased for soleus and gastrocnemius medialis from 10% to 20% [Δ = 1.75 pps (P < 0.001) and Δ = 2.43 pps (P < 0.001), respectively] and from 20% to 30% [Δ = 0.80 pps (P < 0.017) and Δ = 0.92 pps (P = 0.002), respectively]. The repeated-measures correlation identified associations between ΔF and increases in maximal discharge rate for soleus (r = 0.64; P < 0.001) and gastrocnemius medialis (r = 0.77; P < 0.001). An increase in voluntary drive tends to increase PIC strength, which has key implications for the control of force but also for comparisons between muscles or studies when relative force levels might be different. Increases in voluntary descending drive amplify PICs in humans and provide an important spinal mechanism for motor unit discharging, and thus force output modulation.NEW & NOTEWORTHY Animal experiments and computational models have shown that motor neurons can amplify the synaptic input they receive via persistent inward currents. Here we show in humans that this amplification varies proportionally to the magnitude of the voluntary drive to the muscle.

Effect of push-up variations performed with Swiss ball on muscle electromyographic amplitude in trained men: A cross-sectional study.

PURPOSE: To compare the use of three variations of push-ups: traditional (stable surface), performed with hands on the Swiss ball (Swiss ball-hands), and performed with feet on the Swiss ball (Swiss ball-feet) on surface electromyography activity (sEMG) in the agonist and stabilizer muscles. METHODS: Ten trained men (26 ±â€¯5 years, 76.8 ±â€¯8.7 kg, 1.70 ±â€¯0.06 m) performed one experimental protocol within-subjects in a randomized design. Each subject performed one set of 10 of each of the push-up variations (5-min rest between sets). The sEMG activity was assessed for pectoralis major, triceps brachii, anterior deltoids, and rectus abdominis. One-way repeated-measures ANOVA (Bonferroni) compared push-up variations within-muscles (p < 0.05). RESULTS: Pectoralis major sEMG was similar between exercises. Anterior deltoid sEMG activity was greater for stable surface than for Swiss ball-hands (p = 0.001). Triceps brachii sEMG activity was greater during Swiss ball-hands than during stable surface (p = 0.001) and Swiss ball-feet (p = 0.043), and Swiss ball-feet was greater than stable surface (p = 0.001). Rectus abdominis sEMG activity was greater during Swiss ball-hands than during stable surface (p = 0.0001) and Swiss ball-feet (p = 0.036), while Swiss ball-feet was greater than stable surface (p = 0.046). CONCLUSIONS: Push-ups performed with hands on the Swiss ball may be considered an advanced variation that should be used when the goal is to achieve greater challenge of the rectus abdominis and triceps brachii. Novice subjects or those with weakness/injury should perform push-ups with hands on a stable surface, and with progression, push-ups with feet on Swiss ball could be adopted before hands on Swiss ball.

Effect of exercise order with barbell and machine modalities on upper body volume load and myoelectric activity.

The purpose of this study was to investigate the influence of exercise order on volume load (VL) and myoelectric activation (EMG) during the bench press (BP), military press (MP) and close-grip bench press (CGBP) exercises executed with a barbell and Smith machine. Twelve men experienced in resistance training performed four different exercise sessions in randomised order. Each session consisted of four sets of a given exercise order: O1 = CGBP + MP + BP with barbell; O2 = inverse O1 with barbell, O3 = same O1 with Smith Machine; O4 = same O2 with Smith machine. EMG was assessed for the Clavicular head pectoralis major (PMC), anterior deltoid (AD), triceps brachii long head (TBLH) and biceps brachii (BB). Results showed that VL in BP was affected by exercise order, independent of the mode (p < 0.05). However, the CGBP showed higher VL in O1. Moreover, when the BP was positioned last in the sequence (O1 and O3), myoelectric activity was higher for PMC, AD and TBLH (p < 0.05). Findings were similar in the CGBP (PMC and TBLH), but for the AD (Smith machine > barbell, p < 0.05). Therefore, it appears that the order and modes of exercises influence both volume load and myoelectric activation patterns during multiple set of resistance training.

Photobiomodulation Therapy on Physiological and Performance Parameters During Running Tests: Dose-Response Effects.

Dellagrana, RA, Rossato, M, Sakugawa, RL, Baroni, BM, and Diefenthaeler, F. Photobiomodulation therapy on physiological and performance parameters during running tests: Dose-response effects. J Strength Cond Res 32(10): 2807-2815, 2018-This study was aimed at verifying effects of photobiomodulation therapy (PBMT) with different energy doses (15, 30, and 60 J per site) on physiological and performance parameters during running tests. Fifteen male recreational runners participated in a crossover, randomized, double-blind, and placebo-controlled trial. They performed testing protocol in 5 sessions with different treatments: control, placebo, and PBMT with 15, 30, or 60 J per site (14 sites in each lower limb). Physiological and performance variables were assessed during submaximal (at 8 and 9 km·h) and maximal running tests. Photobiomodulation therapy with 30 J significantly improved running economy (RE) at 8 and 9 km·h (3.01%, p=0.008 and 3.03%, p=0.009, respectively), rate of perceived exertion (RPE) at 8 km/h21 (7.86%, p=0.033), velocity at V[Combining Dot Above]O2max (3.07%, p= 0.029), peak of velocity (PV) (1.49%, p=0.035), and total time to exhaustion (TTE) (3.41%, p=0.036) compared with placebo. Photobiomodulation therapy with 15 J improved running economy at 9 km/h21 (2.98%, p=0.025), rate of perceived exertion at 8 km/h21 (4.80%, p=0.010), PV (1.33%, p=0.008), total time to exhaustion (3.06%, p=0.008), and total distance (4.01%, p=0.011) compared with the placebo; whereas PBMT with 60 J only increased RE at 9 km/h21 (3.87%, p=0.024) compared with placebo. All PBMT doses positively affected physiological and/or performance parameters; however, magnitude-based inference reported that PBMT applied with 30 J led to more beneficial effects than 15 and 60 J.

Time Response of Photobiomodulation Therapy on Muscular Fatigue in Humans.

Rossato, M, Dellagrana, RA, Sakugawa, RL, Lazzari, CD, Baroni, BM, and Diefenthaeler, F. Time response of photobiomodulation therapy on muscular fatigue in humans. J Strength Cond Res 32(11): 3285-3293, 2018-The aim of this study was to identify the effects of 2 different time responses on fatigue of knee extensor. Sixteen male volunteers (26 ± 6.0 years, 81 ± 12 kg, and 181 ± 7.4 cm) participated in the study. Participants performed the same protocol in 5 sessions {control, placebo (placebo applied both 6 hours before and immediately before the test), 6 hours before + immediately before (photobiomodulation therapy [PBMT] applied both 6 hours before and immediately before the test), 6 hours before (PBMT applied 6 hours before and placebo applied immediately before the test), and immediately before (placebo applied 6 hours before and PBMT applied immediately before the test)}. Photobiomodulation therapy was applied on knee extensor (9 sites; 30 J per site). Maximal isometric voluntary contraction (MIVC) was assessed before and after an isokinetic fatigue (45 flexion-extension concentric at 180°·s), associated with electromyography (root mean square [RMS] and median of frequency [MF]). For MIVC, there was no treatment × time interaction for all variables. Time effect was observed for peak torque (PT), RMS, and MF. Treatment effect was verified for PT, and 6 hours before + immediately before condition presented higher PT during MIVCpre than control (p = 0.004) and placebo (p = 0.044). The immediately before presented higher PT values than control (p = 0.047). Regarding MIVCpost, the PT for 6 hours before + immediately before presented higher values than control (p = 0.001) and placebo (p = 0.004). Peak torque during MIVC (pre to post) was reduced in 6 hours before + immediately before treatment (26%) compared with control (33%), placebo (29%), and immediately before (32%). The application of PBMT 6 hours + immediately before and immediately before exercise protocol is able to reduce the fatigue.

Effect of cadence on volume and myoelectric activity during agonist-antagonist paired sets (supersets) in the lower body.

The aim of this study was to investigate the effect of cadence on volume load (VLoad) and muscle activity during agonist -antagonist paired sets (APS) in the lower body. Twelve trained men (24.0 ± 3.3 years; 78.3 ± 9.7 kg; 1.77 ± 0.58 m) volunteered to participate in this study. After the 10 maximum repetition test for leg extension and seated leg curl, participants performed three experimental protocols: first protocol (traditional)-three sets of only leg extension (60 bpm); second protocol (fast cadence)-three sets of leg extension, with each set preceded by one set on the seated leg curl with fast cadence (90 bpm); third protocol (slow cadence)-similar to the second, but seated leg curl with slow cadence (40 bpm). The total number of repetitions, VLoad and the electromyography activity for the vastus lateralis, vastus medialis and rectus femoris muscles were recorded. The VLoad was higher during APS than the traditional method, but the cadence (fast or slow) of antagonist exercise did not influence VLoad. Muscle activity of vastus lateralis was higher during traditional protocol; on the other hand, rectus femoris showed higher activity on APS fast cadence protocol, indicating that APS has a different influence on quadriceps components.

Effect of pre-exercise phototherapy applied with different cluster probe sizes on elbow flexor muscle fatigue.

Phototherapy has been used for reducing muscle fatigue. In view of the various types of phototherapy cluster probes available in the market, the purpose of this study was to compare the effects of a similar phototherapy dosage with two different cluster probes on elbow flexor muscle fatigue: small cluster probe (SC = 9 diodes; 7.5 cm(2)) vs. large cluster probe (LC = 33 diodes; 30.2 cm(2)). Ten physically active male aged 18-35 years participate in a randomized, crossover, double-blind, placebo-controlled trial, which each participant was submitted to the same testing protocol in four sessions (separated by at least 48 h) with different treatments: LC-phototherapy, SC-phototherapy, LC-placebo, and SC-placebo. The elbow flexion maximal isometric voluntary contraction (MIVC) was performed before and after a fatigue protocol (60 % of MIVC until exhaustion). Electromyography (EMG) of the biceps brachii muscle was collected during all testing procedure. Phototherapy with dose of 60 J per muscle [LC: 33 diodes = 5 lasers (850 nm), 12 LEDs (670 nm), 8 LEDs (880 nm), and 8 LEDs (950 nm); SC: 9 diodes = 5 lasers (850 nm) and 4 LEDs (670 nm)] or placebo applications occurred before fatigue protocol. Two-way ANOVA (treatment and time factors) and one-way ANOVA were used, followed by LSD post hoc. Time to exhaustion was significantly higher in active LC (15 %; p = 0.031) and SC (14 %; p = 0.038) in comparison with their respective placebo treatments, without differences between LC and SC (p > 0.05) or between placebo conditions (p > 0.05). This larger exercise tolerance in phototherapy conditions was not accompanied by a higher decrement in the volunteers' maximal strength capacity (11-15 %; p > 0.05 for all). EMG signals presented no difference between the four condition tested here. In both large and small cluster probes (according parameters tested in this study) led to reduced fatigue in elbow flexor muscles, without difference between them.